Tank J.L.

Can uptake length in strams be determined by nutrient addition experiments? Results from an interbiome comparison study

Mulholland, P. J; Tanks, J. L.; Webster, J. R.; Bowden, W. B.; Dodds, W. K; Gregory, S. V.; Grimm, N. B; Meriam, J. L.; Meyer, J. L.; Peterson, B. J.; Valett, H. M.; Wollheim, W. M. 2002. Can uptake length in strams be determined by nutrient addition experiments? Results from an interbiome comparison study. J. N. Am. Bethol. Soc. 2002, 21(4): 544-560.

Nutrient uptake length is an important parnmeter tor quantifying nutrient cycling in streams. Although nutrient tracer additions are the preierred method for measuring uptake length under ambient nutrient concentrations, short-term nutrient addition experiments have more irequently been used to estimate uptake length in streams. Theoretical analysis of the relationship between uptake length determined by nutrient addition experiments (Sw') and uptake length determined by tracer additions (Sw)predicted that Sw' should be consistently longer than 5,", and that the overestimate of uptake length by Sw( should be related to the level of nutrient addition above ambient concentrations and the degree of nutrient limitation. To test these predictions, we used data irom an interbiorne study of NH,- uptake length in which 15NH,- tracer and short-term NH,-a ddition experiments were performed in 10 streams using a uniform experimental approach. The experimental results largely contirmed the theoretical predictions: sw' was consistently longer than Sw and Sw':Sw ratios were directly related to the level of NH,- addition and to indicatvrs of N limitation. The experimentally derived Sw':Sw, ratios were used with the theoretical results to infer the N limitation status of each stream. Together, the theoretical and experimental results showed the tracer experiments should be used whenever possible to determine nutrient uptake length in streams. Nutrient addition experiments may be useful for comparing uptake lengths between different streams or cliiferent times in the same stream. however, provided that nutrient additions are kept as low as possible and of similar miagnitude.

Characterizing nitrogen dynamics, retention and transport in a tropical rainforest stream using an in situ 15N addition

Merriam, J. L. , McDowell, W. H. , Tank, J. L. , Wollheim, W. M. , Crenshaw, C. L. and Johnson, S. L. (2002), Characterizing nitrogen dynamics, retention and transport in a tropical rainforest stream using an in situ15N addition. Freshwater Biology, 47: 143–160. doi: 10.1046/j.1365-2427.2002.00785.x

1. This study was part of the Lotic Intersite Nitrogen eXperiment (LINX); a series of identical 15NH4 tracer additions to streams throughout North America. 15NH4Cl was added at tracer levels to a Puerto Rican stream for 42 days. Throughout the addition, and for several weeks afterwards, samples were collected to determine the uptake, retention and transformation pathways of nitrogen in the stream. 2. Ammonium uptake was very rapid. Nitrification was immediate, and was a very significant transformation pathway, accounting for over 50% of total NH4 uptake. The large fraction of NH4 uptake accounted for by nitrification (a process that provides energy to the microbes involved) suggests that energy limitation of net primary production, rather than N limitation, drives N dynamics in this stream. 3. There was a slightly increased 15N label in dissolved organic nitrogen (DON) the day after the 15NH4 addition was stopped. This DO15N was < 0.02% of DON concentration in the stream water at the time, suggesting that nearly all of the DON found in-stream is allochthonous, or that in-stream DON production is very slow. 4. Leptophlebiidae and Atya appear to be selectively feeding or selectively assimilating a very highly labelled fraction of the epilithon, as the label found in the consumers became much higher than the label found in the food source. 5. A large spate (>20-fold increase in discharge) surprisingly removed only 37% of in-stream fine benthic organic matter (FBOM), leaves and epilithon. The fraction that was washed out travelled downstream a long distance (>220 m) or was washed onto the stream banks. 6. While uptake of 15NH4 was very rapid, retention was low. Quebrada Bisley retained only 17.9% of the added 15N after 42 days of 15N addition. Most of this was in FBOM and epilithon. Turnover rates for these pools were about 3 weeks. The short turnover times of the primary retention pools suggest that long-term retention (>1 month) is minimal, and is probably the result of N incorporation into shrimp biomass, which accounted for < 1% of the added 15N.
Syndicate content